Description for MOTS-c 10mg.

MOTS-c 10mg Peptide – The Mitochondrial-Derived Peptide Powering Metabolic Resilience & Longevity Research

What if a tiny, 16-amino-acid peptide encoded directly inside the mitochondrial genome could act as a master metabolic regulator—boosting NAD+ salvage, activating AMPK, enhancing fat oxidation, improving insulin sensitivity, protecting against age-related physical decline, and even mimicking some benefits of exercise in laboratory models? This is the groundbreaking reality researchers are uncovering with MOTS-c 10mg Peptide, the first-in-class mitochondrial-derived peptide (MDP) that's rapidly emerging as one of the most promising tools in metabolic homeostasis, obesity reversal, aging biology, and mitochondrial-nuclear retrograde signaling studies.

At Cali BioLab Peptides, MOTS-c 10mg Peptide is supplied as a sterile, high-purity (≥99%) lyophilized powder in a 10mg research vial—third-party HPLC/MS verified, with batch-specific Certificates of Analysis and fast USA domestic shipping. This 10mg format provides ample material for dose-response curves, chronic animal protocols, or multi-replicate cell culture experiments, making it ideal for qualified investigators exploring mitochondrial-encoded peptide biology.

Strict Disclaimer: For laboratory and scientific research use only. Not for human consumption, therapeutic, diagnostic, veterinary, or any non-research application. Strictly intended for qualified researchers conducting in-vitro, ex-vivo, or ethically approved animal model studies. Cali BioLab Peptides maintains full regulatory compliance.

The Experiment That Redefined Exercise-Mimetic Research: Dr. Chang’s High-Fat Diet Mouse Model

In a mitochondrial metabolism and aging lab at a top-tier U.S. university, Dr. Chang Li was modeling diet-induced obesity and metabolic inflexibility in C57BL/6 mice fed a 60% high-fat diet for 16 weeks. Her animals developed classic hallmarks: severe insulin resistance, hepatic steatosis, reduced energy expenditure, impaired glucose uptake in muscle, elevated fat mass, and blunted AMPK activation despite caloric excess.

Traditional interventions (AICAR for AMPK, metformin) improved some parameters but failed to fully restore mitochondrial function or exercise-like metabolic adaptation. Chang had followed the seminal 2015 Lee et al. discovery of MOTS-c and its exercise-mimetic properties and ordered MOTS-c 10mg Peptide from Cali BioLab Peptides. The 10mg vial arrived lyophilized with a COA confirming 99.5% purity and structural integrity.

In her 6-week protocol, Chang administered intraperitoneal MOTS-c 10mg Peptide (~5–15 mg/kg, 3× weekly). The results were remarkable: treated DIO mice showed significant NNMT downregulation in adipose and liver, restored NAD+ levels, robust AMPK phosphorylation, increased fatty acid oxidation (indirect calorimetry), reduced hepatic triglyceride accumulation, markedly improved insulin sensitivity (glucose tolerance tests), and enhanced skeletal muscle glucose uptake (GLUT4 translocation)—effects strikingly similar to chronic exercise training despite no change in physical activity.

Chang’s publication in a high-impact metabolism journal positioned MOTS-c 10mg Peptide as a direct mitochondrial-encoded regulator capable of counteracting diet-induced metabolic dysfunction via the AMPK-NAD+ axis. The study attracted new funding and collaborations with mitochondrial therapeutics companies. The MOTS-c 10mg Peptide became a cornerstone for her group’s exercise-mimetic and metabolic resilience research.

What Exactly Is MOTS-c 10mg Peptide?

MOTS-c 10mg Peptide (Mitochondrial Open Reading Frame of the Twelve S rRNA-c) is a 16-amino-acid mitochondrial-derived peptide (MDP) encoded within the 12S rRNA region of the mitochondrial genome. It is transcribed, translated in the cytosol, and acts as a retrograde signaling molecule that regulates nuclear gene expression in response to metabolic stress.

Key product specifications:

• Quantity: 10mg sterile lyophilized powder per vial—ideal for dose-response curves, chronic administration, or multiple animal/cell cohorts
• Purity: ≥99% (third-party verified by HPLC and Mass Spectrometry)
• Sequence: MRWQEMGYIFYPRKLR (Met-Arg-Trp-Gln-Glu-Met-Gly-Tyr-Ile-Phe-Tyr-Pro-Arg-Lys-Leu-Arg)
• Molecular Formula: C₁₀₁H₁₅₂N₂₈O₂₂S₂
• Molecular Weight: 2174.6 g/mol
• CAS Number: 1627580-64-6
• Form: White, sterile lyophilized powder
• Storage: –20 °C long-term; 2–8 °C after reconstitution with bacteriostatic water or PBS
• COA: Batch-specific analytical report included

In research models, MOTS-c 10mg Peptide translocates to the nucleus under metabolic stress to regulate gene expression via AMPK activation, promoting fatty acid β-oxidation, glucose uptake (GLUT4), and mitochondrial biogenesis while suppressing pro-inflammatory pathways.

Here are professional examples of MOTS-c 10mg Peptide research-grade lyophilized vials in sterile glass packaging, showcasing the clean, high-quality presentation typical for lab use:

How Does MOTS-c 10mg Peptide Work in Biological Systems?

MOTS-c 10mg Peptide functions as a retrograde signaling peptide from mitochondria to nucleus:

• Under metabolic stress (high-fat diet, aging, exercise), MOTS-c is translated in the cytosol and translocates to the nucleus.
• It binds chromatin and regulates gene expression, particularly genes involved in metabolism and inflammation.
• Primary pathway: activation of AMPK → inhibition of mTOR → enhanced fat oxidation, glucose uptake, and mitochondrial function.
• Secondary effects: reduced NNMT activity (preserving NAD+), suppression of inflammatory cytokines, improved insulin signaling.

These actions are dose-dependent and often more pronounced in metabolically stressed or aged models.

Research Applications of MOTS-c 10mg Peptide

MOTS-c 10mg Peptide is applied in:

• Diet-induced obesity and metabolic syndrome reversal models
• Exercise-mimetic studies (metabolic adaptation without physical activity)
• Aging-related physical decline and muscle homeostasis
• Insulin resistance and type 2 diabetes analogs
• Mitochondrial-nuclear retrograde signaling pathways
• NAFLD/non-alcoholic steatohepatitis models
• Neuroprotection in metabolic stress contexts

Key research endpoints:

• Increased fatty acid oxidation & energy expenditure (indirect calorimetry)
• Restored NAD+ levels & AMPK activation (LC-MS/MS, Western blot)
• Improved insulin sensitivity (glucose/insulin tolerance tests)
• Reduced body fat mass & adipocyte size (DEXA, histology)
• Enhanced skeletal muscle glucose uptake (GLUT4 translocation)
• Lowered inflammatory markers (TNF-α, IL-6)

Usage and Reconstitution Guidelines for MOTS-c 10mg Peptide

1. Allow vial to reach room temperature.
2. Add 1–2 mL bacteriostatic water or sterile PBS; gently swirl.
3. Prepare stock solutions (e.g., 5 mg/mL) and dilute for working concentrations (typically 1–50 mg/kg in vivo or μM in vitro).
4. Aliquot immediately → store at –80 °C. Thawed aliquots stable 2–8 °C for days.

Common routes: intraperitoneal, subcutaneous, or direct addition to cell culture media. Use sterile technique.

Frequently Asked Questions About MOTS-c 10mg Peptide

Q: How does MOTS-c 10mg Peptide differ from NAD+ precursors like NMN or NR? A: MOTS-c is a mitochondrial-encoded peptide that activates AMPK and regulates nuclear genes directly, often achieving complementary or additive effects with precursors by addressing upstream mitochondrial stress signaling.

Q: What dosing ranges are common in preclinical literature? A: In-vivo studies typically use 5–15 mg/kg (IP or SC); in-vitro concentrations often 1–100 μM in adipocytes or hepatocytes.

Q: Is MOTS-c 10mg Peptide cell-permeable and stable? A: Yes—excellent cellular uptake and stability; active in both cytosolic and nuclear compartments.

Q: Suitable for muscle or exercise-mimetic models? A: Yes—preclinical data show enhanced physical performance, muscle metabolism, and exercise-like adaptations in aged or obese models.

Q: How to verify batch quality? A: Match the provided COA on the product page.

One Question That Could Shape Your Next Metabolic or Longevity Study

If you could directly activate mitochondrial-nuclear retrograde signaling right now in a metabolic stress or aging model, which endpoint—fat oxidation, insulin sensitivity, NAD+ preservation, or exercise-like performance—would you target first, and what key assay would you use to prove efficacy?

Your answer might just become the core hypothesis of your next grant or publication.

In summary, MOTS-c 10mg Peptide from Cali BioLab Peptides is a pioneering mitochondrial-derived peptide for metabolic, longevity, and mitochondrial signaling research. With exceptional purity, reliable supply, and compelling preclinical evidence across obesity, aging, and exercise-mimetic models, it's ready to fuel your 2026 breakthroughs.

Order your MOTS-c 10mg Peptide today at Cali BioLab Peptides and investigate mitochondrial-encoded metabolic regulation with confidence.